FastQCFastQC Report
Mon 6 Jun 2016
ERR1378415.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1378415.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1001874
Sequences flagged as poor quality0
Sequence length51
%GC40

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGGTTC501.9819854E-631.4985688
CTCGTAT608.1898925E-626.25011642
GAGCGGT651.5222673E-524.2332956
TCGTATG651.5234447E-524.23087943
GACCGTG651.5240337E-524.22966829
CCGTGAC651.5240337E-524.22966831
AGAGCGG702.7003702E-522.5023445
CGTATGC702.7024536E-522.500144
CGTGACT702.7034957E-522.49897632
CGGTTCA702.7034957E-522.4989769
ACCGTGA702.7034957E-522.49897630
CGGAAGA754.5875764E-521.0084821
TATCTCG754.6017172E-521.00009339
AGCGGTT754.6034875E-520.9990467
ATGCCGA754.6034875E-520.99904622
TCTCGTA807.561503E-519.68758841
GAGACCG807.564405E-519.68660527
CACGCCC1151.5955993E-719.56432711
CGCCCGG851.20466466E-418.5285713
ACGCCCG800.001711158916.87423312
Produced by FastQC (version 0.11.5)