FastQCFastQC Report
Mon 6 Jun 2016
ERR1378412.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1378412.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences905431
Sequences flagged as poor quality0
Sequence length51
%GC41

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCGCGTA556.002665E-1140.9089431
CGCGTAT556.002665E-1140.9089432
ACCGCGT601.546141E-1037.4998630
TCTCGTA702.1827873E-1135.35701441
GACCGCG653.6743586E-1034.61525729
AGACCGC751.7025741E-929.9998928
TCGTATG751.7025741E-929.9998943
GCGTATT751.7025741E-929.9998933
CGTATGC856.4774213E-926.47049144
CTCGTAT957.421477E-1026.05253642
ACGCCGG450.001226380124.99990722
ATCTCGT1001.3442332E-924.74990840
CGTATTA1001.3442332E-924.74990834
CGGAAGA1003.6316123E-822.5061321
TATCTCG1003.6423444E-822.49991639
GCGATGG500.0022642922.49991628
GCGGTTC1003.644527E-822.4986748
GAGACCG1056.1045284E-821.42849227
AGCGGTT1056.1079845E-821.4273097
CGGTTCA1301.2278178E-920.768019
Produced by FastQC (version 0.11.5)