FastQCFastQC Report
Mon 6 Jun 2016
ERR1378411.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1378411.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences908012
Sequences flagged as poor quality0
Sequence length51
%GC41

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATCTCG659.094947E-1238.0757639
GACCGGC803.6379788E-1233.74896629
ACCGGCT754.7293724E-1132.99899330
CTCGTAT754.7293724E-1132.99899342
AGACCGG754.7293724E-1132.99899328
CGTATGC801.0004442E-1030.93655244
CGGCTAT801.0004442E-1030.93655232
TCTCGTA903.965397E-1027.49915941
TCTCTCG450.001226574924.99923513
GAGCGGT1001.3442332E-924.749246
TGCCGAG1101.5097612E-1024.54470323
AAGAGCG1052.3592293E-923.57724
GCGGTTC1052.3665052E-923.5707078
CGGTTCA1052.3665052E-923.5707079
AGCGGTT1052.3665052E-923.5707077
TCGTATG1052.3665052E-923.57070743
CGGAAGA1104.0254236E-922.511711
ATCTCGT1104.0508894E-922.49931140
AGAGCGG1156.7666406E-921.521085
GAGACCG1201.1048542E-820.6243727
Produced by FastQC (version 0.11.5)