FastQCFastQC Report
Mon 6 Jun 2016
ERR1378410.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1378410.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences924412
Sequences flagged as poor quality0
Sequence length51
%GC41

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAGCGGT606.4283086E-933.7506876
AGACCGT754.7293724E-1132.9988928
GACCGTT651.4033503E-831.15279829
TCTCGTA651.4033503E-831.15279841
CCGAGAC801.0004442E-1030.93645925
ATCTCGT702.8858267E-828.92759740
GCGGTTC702.8858267E-828.9275978
CGTATGC755.6385034E-826.99909244
CCGTTGA801.0536678E-725.3116531
TGCCGAG801.0536678E-725.3116523
AAGAGCG851.8894752E-723.8278834
AGCGGTT851.8924402E-723.8240177
CGGTTCA851.8934224E-723.8227279
AATGCCG851.8934224E-723.82272721
ACCGTTG851.8934224E-723.82272730
ATGCCGA851.8934224E-723.82272722
GAGACCG903.2865682E-722.49924327
GTATGCC1003.6437996E-822.49924345
AGAGCGG955.528218E-721.3162255
CTCGTAT955.53111E-721.31507142
Produced by FastQC (version 0.11.5)