FastQCFastQC Report
Mon 6 Jun 2016
ERR1378409.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1378409.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1076807
Sequences flagged as poor quality0
Sequence length51
%GC40

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGACCGC653.6743586E-1034.61470428
CTCGTAT708.1490725E-1032.14222742
GACCGCT708.1490725E-1032.14222729
TCTCGTA751.7043931E-929.99941341
CGTATGC903.965397E-1027.4994644
CGGTTCA903.965397E-1027.499469
AGATCGT450.001225648925.0029933
TATCTCG901.1910743E-824.9995139
GCGGTTC1052.3665052E-923.5709678
ACCGCTG1052.3665052E-923.57096730
ATCTCGT1104.0527084E-922.4995640
TCGTATG1104.0527084E-922.4995643
CGGAAGA1201.0999429E-820.6332171
ATGCCGA1201.105218E-820.62459622
CGCTGAT1109.9847966E-820.45414532
CCGCTGA1109.9847966E-820.45414531
GAGACCG1109.9847966E-820.45414527
CCCCGAT550.003936825320.45414524
CCGAGAC1109.9847966E-820.45414525
AGCGGTT1351.9645086E-919.9996077
Produced by FastQC (version 0.11.5)