FastQCFastQC Report
Mon 6 Jun 2016
ERR1378408.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1378408.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1027680
Sequences flagged as poor quality0
Sequence length51
%GC40

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCGTCGA659.094947E-1238.0749531
GACCGTC850.034.40997729
GCGGTTC801.0004442E-1030.9373998
CGATTAT801.0004442E-1030.93589435
CGTCGAT801.0004442E-1030.93589432
TCGATTA901.2732926E-1129.99844434
ACCGTCG852.0372681E-1029.11613530
AGCGGTT903.947207E-1027.501257
TATCTCG903.965397E-1027.49857339
GAGCGGT1004.5474735E-1127.0051676
CTCGTAT957.421477E-1026.0512842
CGGTTCA957.421477E-1026.051289
AGAGCGG1058.367351E-1125.7192065
TCTCGTA1001.3460522E-924.74871441
CGTATGC1052.3683242E-923.57020444
TCGTATG1052.3683242E-923.57020443
AAGAGCG1104.0363375E-922.5075934
GTCGATT1104.0545274E-922.4988333
AGACCGT1551.8189894E-1221.77306428
CGGAAGA1201.0986696E-820.6349741
Produced by FastQC (version 0.11.5)