FastQCFastQC Report
Mon 6 Jun 2016
ERR1378406.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1378406.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences937602
Sequences flagged as poor quality0
Sequence length51
%GC41

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATCTCG551.12668204E-732.72944639
GACCGAC551.12720954E-732.727729
CTCGTAT602.416764E-730.0019942
TCGTATG602.416764E-730.0019943
ACCGACC602.4178917E-730.00039130
ACGTAGA300.005147794729.9987933
GCGGTTC602.4201654E-729.9971928
CGGTTCA602.4201654E-729.9971929
GAGCGGT654.875765E-727.6897166
AGACCGA751.696897E-624.00031328
AGCGGTT751.6984741E-623.9977537
CGACCAT851.8926403E-723.82384132
CGTATGC802.9720704E-622.50149344
GAGACCG903.2851858E-722.50029427
ATGCCGA802.9748244E-622.49909422
TCTCGTA855.027683E-621.17787741
CCGAGAC855.0323306E-621.1756225
AATGCCG855.034657E-621.1744921
AGAGCGG855.034657E-621.174495
CCGACCA1009.050127E-720.25026531
Produced by FastQC (version 0.11.5)