FastQCFastQC Report
Mon 6 Jun 2016
ERR1378404.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1378404.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1044135
Sequences flagged as poor quality0
Sequence length51
%GC43

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACCGGTC250.002107537435.998213
AGACCGT1450.031.03441628
CGTATGC1450.029.48269544
TATCTCG1500.028.50130539
GCGGTTC1700.027.7940588
ATCTCGT1550.027.58190740
CTCGTAT1550.027.58058542
TCTCGTA1550.027.58058541
GACCGTG1700.026.47053129
GAGCGGT1800.026.2499436
AGCGGTT1850.025.5404877
AGAGCGG1850.025.5404875
TCGTATG1700.025.14700543
CGGTTCA1900.024.8671789
GTATGCC1850.023.10805945
CGTGTTG2200.022.49887532
AAGAGCG2150.021.977754
CGGAAGA2100.021.4346851
CCGTGTT2550.020.29310231
CCGAGAC2250.019.99995825
Produced by FastQC (version 0.11.5)