FastQCFastQC Report
Mon 6 Jun 2016
ERR1378401.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1378401.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences901555
Sequences flagged as poor quality0
Sequence length51
%GC40

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATGC850.037.05795344
TCGTATG900.034.9991843
TCTCGTA950.033.1571241
AGACCGT950.033.1571228
CTCGTAT1000.031.49926242
GAGCGGT950.030.7904626
AGCGGTT1050.030.0009617
GAGACCG1050.029.99929827
CCGAGAC1100.028.63569325
ATGCCGA1200.028.12434222
GCGGTTC1150.027.3906638
GACCGTG1150.027.39066329
AATGCCG1150.027.39066321
ATCTCGT1350.024.99941440
AGAGCGG1201.6370905E-1124.375785
CGTGTTC1301.8189894E-1224.23020432
CCGTGTT1353.6379788E-1223.33278831
AATCTCG1353.6379788E-1223.33278839
TGCCGAG1353.6379788E-1223.33278823
AAGAGCG1305.0931703E-1122.501974
Produced by FastQC (version 0.11.5)