FastQCFastQC Report
Mon 6 Jun 2016
ERR1378400.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1378400.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1113284
Sequences flagged as poor quality0
Sequence length51
%GC40

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCGTAT1100.032.72596742
GACCGAA1150.031.30309729
TCGTATG1150.031.30309743
CCGAACT1150.031.30309731
AGCGGTT1250.030.6029017
GAGCGGT1250.030.6029016
CGTATGC1200.029.99880244
TATCTCG1200.029.99880239
AGACCGA1250.028.7988528
GCGGTTC1350.028.3334738
ACCGAAC1300.027.69120430
CGGTTCA1400.027.320349
AAGAGCG1350.026.6727894
AGAGCGG1400.025.7167245
TCTCGTA1400.025.7132641
TGCCGAG1450.024.82659523
ATGCCGA1550.024.67643422
ATCTCGT1600.023.90529440
AATGCCG1650.023.18089321
GAGACCG1700.021.17562527
Produced by FastQC (version 0.11.5)