FastQCFastQC Report
Mon 6 Jun 2016
ERR1378399.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1378399.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1010675
Sequences flagged as poor quality0
Sequence length51
%GC40

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGATATC504.8807124E-835.99770432
CCGATAT458.702882E-734.9977731
ACCGATA458.702882E-734.9977730
GACCGAT501.9822928E-631.49799229
CGCGTAA300.00514857429.99808722
TCTCGTA755.6385034E-826.99961341
GCGGTTC651.5236836E-524.2304238
GAGCGGT754.5971734E-521.0028156
AGACCGA754.60419E-520.99866128
CGTATGC654.2381528E-420.76893444
CTCGTAT654.2381528E-420.76893442
CATCTCG654.2381528E-420.76893439
CGGTTCA807.565551E-519.6862459
ATGCCGA807.565551E-519.68624522
CTTCGTT1757.2759576E-1219.2940312
ACGGGAT1454.774847E-918.61950313
CGGAAGA851.1998296E-418.5383191
AGCGGTT851.2039339E-418.5300647
ATCTCGT1002.0464897E-517.99974340
CCGAGAC901.8663058E-417.49888425
Produced by FastQC (version 0.11.5)