FastQCFastQC Report
Mon 6 Jun 2016
ERR1378397.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1378397.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences614928
Sequences flagged as poor quality0
Sequence length51
%GC42

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGGAAGAGCGGTTCAGCAGGAATGCCGAGACCGTAACCTATCTCGTATGCC10130.16473473317201365Illumina Paired End PCR Primer 2 (96% over 33bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCGTAT1100.038.8618842
CGTAACC1150.037.17223432
CCGTAAC1200.035.6233931
TCGTATG1200.035.6233943
AAGAGCG1300.034.62794
CGTATGC1250.034.19845244
AGACCGT1350.031.66523728
ACCGTAA1350.031.66523730
CCGAGAC1450.031.03308125
GTATGCC1400.030.53433645
TCTCGTA1400.030.53433641
GTCGAAT300.005146555629.99864418
TATCTCG1500.029.99864439
GAGACCG1450.029.48142627
GCGGTTC1550.029.0309478
GACCGTA1500.028.49871329
GTAACCT1550.027.579433
CGGTTCA1700.026.469399
AGCGGTT1700.026.469397
ATCTCGT1650.025.9079240
Produced by FastQC (version 0.11.5)