FastQCFastQC Report
Mon 6 Jun 2016
ERR1378396.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1378396.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1115272
Sequences flagged as poor quality0
Sequence length51
%GC41

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCGCTTA458.703755E-734.9978731
CGTATGC501.9824893E-631.49808744
TCTCGTA554.1667627E-628.63462341
AGACCGC554.1667627E-628.63462328
GCGGTTC608.192277E-626.2495828
CTCGTAT709.3120616E-725.71272342
GACCGCT651.52441535E-524.22929829
CGCTTAC651.52441535E-524.22929832
TCGTATG702.7041691E-522.49863243
AATGCCG855.034639E-621.17518221
ATGCGAT550.003937851620.45330227
AAGAGCG1009.0169124E-720.258764
GGCGCAA600.00651266418.7488613
ATCTCGT851.2045478E-418.52911640
ACCGCTT851.2049619E-418.52828430
CCGAGAC1002.0476165E-517.99890725
GAGCGGT901.8639241E-417.5020756
AGCGGTT901.8658425E-417.4997227
CGGCTAA1305.785878E-717.30664317
ACGGGAT1358.587285E-716.66565513
Produced by FastQC (version 0.11.5)