FastQCFastQC Report
Mon 6 Jun 2016
ERR1378395.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1378395.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences995939
Sequences flagged as poor quality0
Sequence length51
%GC40

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGATTAC650.041.53929532
AGCGGTT751.8189894E-1235.997117
GACCGAT900.035.000729
CCGATTA803.6379788E-1233.7506831
TCTCGTA803.6379788E-1233.7506841
ACCGATT900.032.50065230
AGACCGA900.032.50065228
GAGCGGT855.456968E-1231.7637486
CGGTTCA855.456968E-1231.7621549
AGAGCGG1001.8189894E-1229.2491195
AATGCCG1001.8189894E-1229.24765221
CGTATGC952.3646862E-1128.42162144
GCGGTTC952.3646862E-1128.418778
CTCGTAT1004.5474735E-1127.00054442
TGCCGAG1004.5474735E-1127.00054423
CCGAGAC1159.094947E-1225.43529525
GAGACCG1101.5097612E-1024.54594827
TCGTATG1101.5097612E-1024.54594843
AAGAGCG1201.6370905E-1124.377944
CGGAAGA1358.367351E-1121.674721
Produced by FastQC (version 0.11.5)