FastQCFastQC Report
Mon 6 Jun 2016
ERR1378394.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1378394.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1009286
Sequences flagged as poor quality0
Sequence length51
%GC40

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCGTAT554.1638486E-628.6367442
GACCGTT551.3679187E-424.54577829
AACGGGT651.5239408E-524.22988912
CCGTTGA702.7022948E-522.500331
AGACCGT602.466999E-422.50029828
ACCGTTG654.23736E-420.76950530
ACGCCCG550.003937039520.45380212
CGCCCGG1009.0558206E-720.24926413
ATCTCGT908.248668E-620.00026540
CGTATGC706.98204E-419.2859744
CGTTGAC706.98204E-419.2859732
AGAGCGG706.98204E-419.285975
CGTGAGC2000.019.1271481
CCGGCTA951.31598445E-518.9466816
CATGCGC600.00650573918.7521061
AGCGGTT750.001109997918.0002377
TCTCGTA750.001109997918.00023741
GAGACCG1205.1711904E-616.87522327
CCTTCGT1752.6848284E-916.7161621
GCCCGGC2057.4578566E-1116.46281614
Produced by FastQC (version 0.11.5)