FastQCFastQC Report
Mon 6 Jun 2016
ERR1378393.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1378393.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1042410
Sequences flagged as poor quality0
Sequence length51
%GC40

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCGTAT356.248598E-638.5706542
ACCGTTC501.981698E-631.49936130
TCTCGTA453.491019E-529.99939241
GACCGTT507.158758E-526.99945329
CGTTCAC507.158758E-526.99945332
GAGACCG551.3682713E-424.54495827
AGACCGT551.3682713E-424.54495828
CCGTTCA551.3682713E-424.54495831
GCGGTTC702.704106E-522.4984658
CGGTTCA754.6045265E-520.9985689
TCGTATG654.238446E-420.7688143
CGTGAGC1651.8189894E-1220.4629651
TACGGAG550.003931291420.4590384
GAGCGGT851.2031633E-418.5317046
ATCTCGT851.2044916E-418.52903640
AGCGGTT750.001109565718.0013627
CGTATGC750.001110280317.99963444
CGGGATT1253.835121E-717.99877414
CGGAGGT1401.2546861E-616.07110433
GCGGAGG1552.0659536E-715.9674232
Produced by FastQC (version 0.11.5)