FastQCFastQC Report
Mon 6 Jun 2016
ERR1378391.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1378391.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1314464
Sequences flagged as poor quality0
Sequence length51
%GC40

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTCGTA702.8874638E-828.92800741
CTCGTAT803.3978722E-928.12445442
GACCGTC755.6415956E-826.99947529
CCGTCAA755.6415956E-826.99947531
CGTATGC801.0542317E-725.3120144
CGGTTCA801.0542317E-725.312019
GCGGTTC851.8944411E-723.8230678
CGTCAAC851.8944411E-723.82306732
TATCTCG903.2882963E-722.49956139
GAGCGGT955.5319833E-721.3161856
AGACCGT1009.05864E-720.24960528
ACCGTCA1009.05864E-720.24960530
AAGAGCG1051.4445795E-619.287544
TCGTATG1051.4461602E-619.2853443
AGCGGTT1153.450068E-617.6083557
CCGAGAC1153.450068E-617.60835525
GAGACCG1459.672658E-817.06863427
ATCTCGT1205.175196E-616.87467240
ATGCCGA1358.5864485E-716.66634222
AATGCCG1257.6292436E-616.19968421
Produced by FastQC (version 0.11.5)