FastQCFastQC Report
Mon 6 Jun 2016
ERR1378388.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1378388.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences893004
Sequences flagged as poor quality0
Sequence length51
%GC42

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGGAAGAGCGGTTCAGCAGGAATGCCGAGACCGCATTACATCTCGTATGCC18690.2092935753927194Illumina Paired End PCR Primer 2 (96% over 33bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCATTA2150.040.81349632
GACCGCA2300.037.17349629
AGACCGC2300.037.17349628
CGTATGC2250.036.99958444
CTCGTAT2300.036.19524442
TCTCGTA2350.035.42513741
AGAGCGG2450.034.897575
TCGTATG2400.034.6871143
ACCGCAT2600.033.74962230
AGCGGTT2550.033.5290377
CCGAGAC2550.033.52903725
GCGGTTC2600.032.8842478
CGGTTCA2600.032.8842479
GAGCGGT2600.032.8842476
AATGCCG2650.032.2637921
CCGCATT2750.031.90873331
GAGACCG2700.031.6663127
ATCTCGT2650.031.41474240
CGGAAGA2850.030.0097451
AAGAGCG2900.029.484084
Produced by FastQC (version 0.11.5)