FastQCFastQC Report
Mon 6 Jun 2016
ERR1378387.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1378387.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1310660
Sequences flagged as poor quality0
Sequence length51
%GC40

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGGACC250.002107128536.00030545
TCGTATG1400.033.75028643
GCGGTTC1400.033.7498
CGTATGC1450.032.58648344
TCTCGTA1400.032.1431341
CGTTGTA1400.032.14190332
GACCGTT1400.032.14190329
CTCGTAT1550.030.4841342
ATCTCGT1500.030.00025440
GCGAGTC300.00514730630.00025440
CGGTTCA1650.029.9991119
GAGCGGT1600.029.53156
CCGTTGT1550.029.03139931
AGACCGT1650.028.63551528
ACCGTTG1600.028.12416530
CATCTCG1650.027.27295939
GAGACCG1750.026.99919927
AGCGGTT1850.025.5397837
ATGCCGA1850.025.53978322
AGAGCGG1900.024.8686335
Produced by FastQC (version 0.11.5)