FastQCFastQC Report
Mon 6 Jun 2016
ERR1378383.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1378383.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences993826
Sequences flagged as poor quality0
Sequence length51
%GC41

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTCGTA602.4179826E-730.00063541
AGCGGTT651.5232308E-524.2312837
GAGCGGT651.5232308E-524.2312836
CTCGTAT802.973533E-622.50047942
GACCGCT802.9748335E-622.49934629
AGACCGC802.9748335E-622.49934628
TATCTCG754.6010726E-521.00044639
AGAGCGG754.6010726E-521.0004465
ACCGCTT754.602857E-520.99939230
AACGGGT550.00393683220.4539512
CGTATGC807.557525E-519.6889144
CCGCTTG807.5633725E-519.68692831
CGGAAGA1051.4427842E-619.2890361
CCTTCGT1302.7655915E-819.0417391
ATCTCGT1153.447014E-617.6090740
AAGAGCG901.8636188E-417.5021324
GCGGTTC901.8657713E-417.499498
CGGTTCA952.8194976E-416.5784669
TCGTATG1004.1656592E-415.75033543
CGTTGGA1602.9610783E-715.4690795
Produced by FastQC (version 0.11.5)