FastQCFastQC Report
Mon 6 Jun 2016
ERR1378382.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1378382.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences756147
Sequences flagged as poor quality0
Sequence length51
%GC40

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAGCGGT751.8189894E-1236.0017976
TCTCGTA751.8189894E-1235.9994241
AGAGCGG754.7293724E-1133.0016485
CTCGTAT754.7293724E-1132.9994742
AGACCGT754.7293724E-1132.9994728
TCGTATG754.7293724E-1132.9994743
AAGAGCG801.0004442E-1030.9390474
GACCGTC801.0004442E-1030.93700229
AGCGGTT1001.8189894E-1229.2495277
CGGAAGA852.0190782E-1029.1268081
ACCGTCT952.3646862E-1128.42059530
CGGTTCA903.947207E-1027.4995569
AATGCCG903.947207E-1027.49955621
TGCCGAG903.947207E-1027.49955623
GCGGTTC957.403287E-1026.0522128
GAGACCG957.403287E-1026.05221227
ATGCCGA957.403287E-1026.05221222
CGTATGC1058.367351E-1125.71387144
CCGAGAC1104.0472514E-922.49963625
ATCTCGT1257.512426E-1021.59965140
Produced by FastQC (version 0.11.5)