FastQCFastQC Report
Mon 6 Jun 2016
ERR1378375.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1378375.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1354582
Sequences flagged as poor quality0
Sequence length51
%GC40

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GACCGTA801.0004442E-1030.93628329
TCTCGTA856.4846972E-926.47052441
GCGGTTC901.1919838E-824.9990148
CGTATGC1001.3460522E-924.74993944
GAGCGGT952.1145752E-823.68596
ATCTCGT952.1162123E-823.68415340
CGGTTCA952.1169399E-823.6832779
AGACCGT952.1169399E-823.68327728
ACCGTAA952.1169399E-823.68327730
CTCGTAT1104.0545274E-922.49994542
AGCGGTT1003.6456186E-822.4999457
CGTAAGT1056.11235E-821.42772732
AGAGCGG1109.9798854E-820.4560075
CGGAAGA1009.0255344E-720.2574271
AAGAGCG1251.7649654E-819.8028764
ATGCCGA1251.7687853E-819.7992222
CCGTAAG1151.596527E-719.56444731
TCGTATG1302.7734131E-819.03841443
CCGAGAC1202.4985093E-718.74926225
AATGCCG1253.8360668E-717.9992921
Produced by FastQC (version 0.11.5)