FastQCFastQC Report
Mon 6 Jun 2016
ERR1378374.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1378374.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences548782
Sequences flagged as poor quality0
Sequence length51
%GC41

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCGTAT451.925946E-839.99797442
TCGTTGT250.002106674235.9981841
CGTATGC606.4228516E-933.74829544
TCTCGTA551.126391E-732.72561641
GAGCGGT651.39898475E-831.157956
AGACCGA751.7007551E-929.99848228
AAGAGCG702.8716386E-828.937654
AGCGGTT702.8767317E-828.9323797
GCGGTTC702.8792783E-828.9297438
CGGAAGA803.3724064E-928.1363951
ACCGAAC654.867725E-727.69090730
TCGTATG755.6306817E-826.99863243
CGAACAT709.2968E-725.71298432
GACCGAA709.2968E-725.71298429
CCGAACA709.2968E-725.71298431
AGAGCGG851.8874925E-723.8266665
AATGCCG1204.5110937E-1022.49886321
GAGACCG802.971361E-622.49886327
CCGAGAC802.971361E-622.49886325
CGGTTCA903.2820572E-722.4988619
Produced by FastQC (version 0.11.5)