FastQCFastQC Report
Mon 6 Jun 2016
ERR1378373.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1378373.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1175940
Sequences flagged as poor quality0
Sequence length51
%GC40

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GACCGGT754.7293724E-1132.9995629
CGTATGC803.3960532E-928.1246344
CCGGTAA903.965397E-1027.49963831
CGGTTCA1004.5474735E-1126.9984939
AAGAGCG856.4683263E-926.4758684
CTCGTAT856.4828782E-926.4702442
AATGCCG856.486516E-926.46911421
AGACCGG957.421477E-1026.05228628
GAGCGGT901.1907105E-825.0007326
ACCGGTA901.1912562E-824.9996730
TCTCGTA901.1912562E-824.9996741
ACGCCCG450.001226970324.99860612
ATGCCGA901.1918019E-824.99860622
CGGTAAT952.1158485E-823.68389532
ATCTCGT1204.5110937E-1022.49970240
CGGAAGA1358.367351E-1121.6774431
CCGAGAC1201.1059456E-820.62385225
TGCCGAG1201.1059456E-820.62385223
AGCGGTT1109.98607E-820.4542757
TCGTATG1109.98607E-820.45427543
Produced by FastQC (version 0.11.5)