FastQCFastQC Report
Mon 6 Jun 2016
ERR1378371.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1378371.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences530641
Sequences flagged as poor quality0
Sequence length51
%GC41

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGAGCGGTTCAGCAGGAATGCCGAGATCGGAAGAGCGGTTCAGCAGGAAT32340.6094515877966459Illumina Paired End PCR Primer 2 (96% over 30bp)
CGGAAGAGCGGTTCAGCAGGAATGCCGAGACCGGCACAAATCTCGTATGCC30510.5749649951662235Illumina Paired End PCR Primer 2 (96% over 33bp)
AAGAGCGGTTCAGCAGGAATGCCGAGACCGGCACAAATCTCGTATGCCGTC25530.48111623489327054Illumina Paired End PCR Primer 2 (96% over 30bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCCG2850.041.83816543
ATGCCGT3100.037.7383844
TGCCGTC3150.037.1393645
ATCGGAA4000.036.55905527
GATCGGA4200.035.88947326
TCGGAAG4250.035.46724728
AGATCGG4350.034.65190525
CGAGATC4800.031.4032923
CCGAGAT4800.030.93458422
GAGATCG5000.030.1471624
CGTATGC6550.025.07397344
AGACCGG6600.024.8840228
GACCGGC6700.024.51261529
GAGACCG6700.024.51261527
CTCGTAT6750.024.33104142
ATCTCGT6700.024.17682640
TCGTATG6800.024.15213443
TCTCGTA6750.023.9977441
ACCGGCA6850.023.97584330
AATCTCG6800.023.82128339
Produced by FastQC (version 0.11.5)