FastQCFastQC Report
Mon 6 Jun 2016
ERR1378370.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1378370.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences544415
Sequences flagged as poor quality0
Sequence length51
%GC41

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGAGCGGTTCAGCAGGAATGCCGAGATCGGAAGAGCGGTTCAGCAGGAAT21400.3930824830322456Illumina Paired End PCR Primer 2 (96% over 30bp)
AAGAGCGGTTCAGCAGGAATGCCGAGACCGGAACACATCTCGTATGCCGTC16960.31152705197321895Illumina Paired End PCR Primer 2 (96% over 30bp)
CGGAAGAGCGGTTCAGCAGGAATGCCGAGACCGGAACACATCTCGTATGCC16000.29389344525775374Illumina Paired End PCR Primer 2 (96% over 33bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCCG2100.039.6384943
TGTTCGC250.00210727635.99603329
ATGCCGT2250.035.9960344
TGCCGTC2300.035.2135145
ATCGGAA3100.032.6576927
GATCGGA3150.032.13931326
AGATCGG3300.030.67843625
TCGGAAG3300.030.67843628
CGGTTAA300.0051476829.99669323
CGAGATC3850.027.46450423
GAGATCG3850.027.46450424
CCGAGAT4000.026.99702522
AATGCCG7200.026.87203818
ATGCCGA7250.026.68671219
TGCCGAG7350.026.62971920
TCTCGTA3300.025.22449338
GCCGAGA7650.024.99724421
GACCGGA3350.024.84800526
CTCGTAT3400.024.48259539
ATCTCGT3500.024.42587937
Produced by FastQC (version 0.11.5)