FastQCFastQC Report
Mon 6 Jun 2016
ERR1378369.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1378369.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences614691
Sequences flagged as poor quality0
Sequence length51
%GC41

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGAGCGGTTCAGCAGGAATGCCGAGATCGGAAGAGCGGTTCAGCAGGAAT43040.7001892007528986Illumina Paired End PCR Primer 2 (96% over 30bp)
CGGAAGAGCGGTTCAGCAGGAATGCCGAGACCGGGTAACATCTCGTATGCC33850.550683188789164Illumina Paired End PCR Primer 2 (96% over 33bp)
AAGAGCGGTTCAGCAGGAATGCCGAGACCGGGTAACATCTCGTATGCCGTC31540.5131033315926213Illumina Paired End PCR Primer 2 (96% over 30bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCCG4400.036.30150243
ATGCCGT4500.035.99472844
TGCCGTC4450.035.8936245
AGATCGG6050.032.7224825
ATCGGAA6050.032.7224827
GATCGGA6100.032.4542626
TCGGAAG6400.031.98750328
CCGAGAT6300.031.4239722
CGAGATC6300.031.4239723
GAGATCG6750.029.32903724
AATGCCG14350.025.86729218
ATGCCGA14600.025.42435819
TGCCGAG14950.024.82914220
ACCGCGC551.3685509E-424.541869
GAATGCC15250.024.34069817
GCCGAGA14950.024.22722221
GGAATGC16650.022.29403316
AGGAATG17300.021.45639615
CGGGTAA7650.021.1733729
CCGGGTA7600.021.0166628
Produced by FastQC (version 0.11.5)