FastQCFastQC Report
Mon 6 Jun 2016
ERR1378368.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1378368.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences401540
Sequences flagged as poor quality0
Sequence length51
%GC42

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGAGCGGTTCAGCAGGAATGCCGAGATCGGAAGAGCGGTTCAGCAGGAAT39570.9854559944214772Illumina Paired End PCR Primer 2 (96% over 30bp)
CGGAAGAGCGGTTCAGCAGGAATGCCGAGACCGGCTAAGATCTCGTATGCC35350.8803606116451661Illumina Paired End PCR Primer 2 (96% over 33bp)
AAGAGCGGTTCAGCAGGAATGCCGAGACCGGCTAAGATCTCGTATGCCGTC27650.6885988942571101Illumina Paired End PCR Primer 2 (96% over 30bp)
TTCAGCAGGAATGCCGAGATCGGAAGAGCGGTTCAGCAGGAATGCCGAGAC5610.13971210838272652Illumina Paired End PCR Primer 2 (100% over 35bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCCG3250.040.15406843
ATGCCGT3600.036.250244
TGCCGTC3650.035.75362445
GAGATCG5500.035.58667424
AGATCGG5550.035.26607525
ATCGGAA5800.033.74598727
GATCGGA5950.033.2733526
TCGGAAG6100.033.1927728
CCGAGAT5900.033.1740222
CGAGATC6100.032.08634623
CGTTGGA507.110442E-527.0203425
CGTATGC7800.024.8078344
TCGTATG7800.024.8078343
GTATGCC7850.024.64981745
ACCGGCT7800.024.51631430
GAGACCG7900.024.49075727
AGACCGG7850.024.36015928
CTCGTAT7950.024.33672542
TCTCGTA7950.024.33672541
CCGGCTA7900.024.2059831
Produced by FastQC (version 0.11.5)