FastQCFastQC Report
Mon 6 Jun 2016
ERR1378367.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1378367.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences954559
Sequences flagged as poor quality0
Sequence length51
%GC41

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGGAAGAGCGGTTCAGCAGGAATGCCGAGACCGGTCAAGATCTCGTATGCC35470.3715852032194972Illumina Paired End PCR Primer 2 (97% over 36bp)
AAGAGCGGTTCAGCAGGAATGCCGAGATCGGAAGAGCGGTTCAGCAGGAAT34540.36184248433046046Illumina Paired End PCR Primer 2 (96% over 30bp)
AAGAGCGGTTCAGCAGGAATGCCGAGACCGGTCAAGATCTCGTATGCCGTC27390.2869387853448556Illumina Paired End PCR Primer 2 (96% over 33bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGCCGTC3500.036.63961845
TATGCCG3550.036.12356643
AGATCGG4700.033.0289925
ATGCCGT4000.032.05966644
GATCGGA4850.032.00747726
ATCGGAA4950.031.81536727
TCGGAAG5200.030.71843728
CCGAGAT6250.024.83780122
GAGATCG6250.024.83780124
CGAGATC6400.024.25566523
AATGCCG12200.023.6044718
ATGCCGA12250.023.50812319
TGCCGAG12350.023.49994720
GAATGCC13600.021.17459717
ACCGGTC6650.020.97558630
GACCGGT6800.020.5128929
AGACCGG6800.020.5128928
CCGGTCA6700.020.48326331
CGGTCAA6750.020.33153532
GCCGAGA13850.020.30506321
Produced by FastQC (version 0.11.5)