FastQCFastQC Report
Mon 6 Jun 2016
ERR1378365.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1378365.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1040442
Sequences flagged as poor quality0
Sequence length51
%GC41

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGGAAGAGCGGTTCAGCAGGAATGCCGAGACCGGACAACATCTCGTATGCC38450.36955447780846984Illumina Paired End PCR Primer 2 (96% over 33bp)
AAGAGCGGTTCAGCAGGAATGCCGAGATCGGAAGAGCGGTTCAGCAGGAAT33870.32553472466509425Illumina Paired End PCR Primer 2 (96% over 30bp)
AAGAGCGGTTCAGCAGGAATGCCGAGACCGGACAACATCTCGTATGCCGTC25730.24729874418756645Illumina Paired End PCR Primer 2 (96% over 30bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCCG4150.038.48871643
ATGCCGT4500.035.49514844
TGCCGTC4600.034.72351545
GATCGGA5250.032.56697526
ATCGGAA5250.032.13846227
AGATCGG5500.030.67762425
TCGGAAG5650.030.26134728
CGATCCC300.00515047929.995924
CCGAGAT6400.026.71509722
CGAGATC6400.026.71509723
GAGATCG6450.026.50800324
ATGCCGA13750.024.2148719
AATGCCG13950.023.86770418
TGCCGAG14000.023.78246320
GCCGAGA15150.021.828721
GAATGCC15400.021.6204217
CGGACAA7700.021.32825332
ACCGGAC7850.020.92070830
GGAATGC16550.020.52589616
CCGGACA8050.020.40093831
Produced by FastQC (version 0.11.5)