FastQCFastQC Report
Mon 6 Jun 2016
ERR1378364.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1378364.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences973766
Sequences flagged as poor quality0
Sequence length51
%GC41

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGAGCGGTTCAGCAGGAATGCCGAGATCGGAAGAGCGGTTCAGCAGGAAT47400.48676992213735126Illumina Paired End PCR Primer 2 (96% over 30bp)
AAGAGCGGTTCAGCAGGAATGCCGAGACCGTCGTTAATCTCGTATGCCGTC35130.3607642903942015Illumina Paired End PCR Primer 2 (96% over 30bp)
CGGAAGAGCGGTTCAGCAGGAATGCCGAGACCGTCGTTAATCTCGTATGCC33050.3394039225029422Illumina Paired End PCR Primer 2 (96% over 33bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATGCCGT4400.038.34833544
TATGCCG4500.037.49614743
TGCCGTC4900.034.43523845
GATCGGA6250.033.47656226
AGATCGG6300.033.21087325
ATCGGAA6350.032.9493727
TCGGAAG6800.032.09229328
CCGAGAT7200.029.37198422
CGAGATC7250.028.85910423
GAGATCG7900.026.76940224
ATGCCGA15100.025.92448819
TGCCGAG15100.025.92448820
AATGCCG15400.025.41946818
GCCGAGA16150.023.68177821
GAATGCC16900.023.29642317
CCGTCGT7950.021.79021528
CGTCGTT8050.021.5195329
GGAATGC18300.021.51418316
GTCGTTA8100.021.38669230
AGACCGT8150.021.25548625
Produced by FastQC (version 0.11.5)