FastQCFastQC Report
Mon 6 Jun 2016
ERR1378363.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1378363.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences932289
Sequences flagged as poor quality0
Sequence length51
%GC41

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGAGCGGTTCAGCAGGAATGCCGAGATCGGAAGAGCGGTTCAGCAGGAAT29590.31739085197830286Illumina Paired End PCR Primer 2 (96% over 30bp)
AAGAGCGGTTCAGCAGGAATGCCGAGACCGTAGTTCATCTCGTATGCCGTC20250.2172073251963715Illumina Paired End PCR Primer 2 (96% over 30bp)
CGGAAGAGCGGTTCAGCAGGAATGCCGAGACCGTAGTTCATCTCGTATGCC13620.1460920379839299Illumina Paired End PCR Primer 2 (96% over 33bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCCG2450.037.6485343
ATGCCGT2600.035.476544
AGATCGG4400.034.7685425
ATCGGAA4300.034.53072427
TGCCGTC2800.033.74593445
GATCGGA4700.032.5492726
TCGGAAG4750.032.2066528
ATGCCGA8900.028.56397819
CCGAGAT5650.028.27093122
AATGCCG9150.027.53766818
TGCCGAG9250.027.23996420
CGAGATC5950.026.84550523
GAGATCG5900.026.691724
GCCGAGA10350.024.34489621
CGGAAGA6500.023.53562729
CCGTAGT4250.022.23261828
CTCGTAT4150.022.22623839
GAATGCC11400.022.10260217
GACCGTA4200.021.96164126
TCGTATG4350.021.72152340
Produced by FastQC (version 0.11.5)