FastQCFastQC Report
Mon 6 Jun 2016
ERR1378362.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1378362.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences694569
Sequences flagged as poor quality0
Sequence length51
%GC41

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGAGCGGTTCAGCAGGAATGCCGAGATCGGAAGAGCGGTTCAGCAGGAAT43150.6212485728559726Illumina Paired End PCR Primer 2 (96% over 30bp)
AAGAGCGGTTCAGCAGGAATGCCGAGACCGGTATTCATCTCGTATGCCGTC30960.4457440513469504Illumina Paired End PCR Primer 2 (96% over 32bp)
CGGAAGAGCGGTTCAGCAGGAATGCCGAGACCGGTATTCATCTCGTATGCC24650.3548963457914188Illumina Paired End PCR Primer 2 (97% over 35bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGCCGTC3850.040.3200345
ATGCCGT3950.039.29927444
TATGCCG4100.037.8614943
AGATCGG5900.034.3180825
GATCGGA5950.034.02969426
ATCGGAA5950.033.65158527
TCGGAAG6250.033.11618428
CCGAGAT6350.031.8860922
CGAGATC6500.031.15025723
GAGATCG6600.031.01915424
AATGCCG13250.027.84584818
ATGCCGA13250.027.84584819
TGCCGAG13450.027.43178420
GCCGAGA13250.027.33647321
GAATGCC14000.026.35410717
GGAATGC14150.026.07473616
GACCGGT6300.025.71132526
CCGGTAT6300.025.71132528
ACCGGTA6400.025.66110627
CTCGTAT6350.025.15458139
Produced by FastQC (version 0.11.5)