FastQCFastQC Report
Mon 6 Jun 2016
ERR1378361.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1378361.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences740878
Sequences flagged as poor quality0
Sequence length51
%GC41

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGAGCGGTTCAGCAGGAATGCCGAGATCGGAAGAGCGGTTCAGCAGGAAT28060.37873981951144453Illumina Paired End PCR Primer 2 (96% over 30bp)
CGGAAGAGCGGTTCAGCAGGAATGCCGAGACCGCCATTAATCTCGTATGCC21020.2837174271607471Illumina Paired End PCR Primer 2 (96% over 33bp)
AAGAGCGGTTCAGCAGGAATGCCGAGACCGCCATTAATCTCGTATGCCGTC20970.28304255221507457Illumina Paired End PCR Primer 2 (96% over 30bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCCG3250.038.07309743
ATGCCGT3400.036.39340244
TGCCGTC3400.036.39340245
GATCGGA3650.032.05157526
AGATCGG3700.031.61844425
ATCGGAA3800.030.7863827
TCGGAAG4200.029.46132728
CGAGATC4350.026.8938523
GAGATCG4450.026.28949224
ATGCCGA10000.025.19746819
AATGCCG10100.024.94798918
TGCCGAG10200.024.92396420
CCGAGAT4800.024.84125322
GAATGCC11200.022.6986117
CGCCATT6000.022.4977429
GCCGAGA10950.022.39500821
AGACCGC5550.022.29505725
CTCGTAT5600.022.09599339
CGTATGC5750.021.51957741
TCTCGTA5750.021.51957738
Produced by FastQC (version 0.11.5)