FastQCFastQC Report
Mon 6 Jun 2016
ERR1378360.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1378360.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences869750
Sequences flagged as poor quality0
Sequence length51
%GC41

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGGAAGAGCGGTTCAGCAGGAATGCCGAGACCGCAATTCATCTCGTATGCC29220.33595860879563094Illumina Paired End PCR Primer 2 (96% over 33bp)
AAGAGCGGTTCAGCAGGAATGCCGAGATCGGAAGAGCGGTTCAGCAGGAAT23520.2704225352112676Illumina Paired End PCR Primer 2 (96% over 30bp)
AAGAGCGGTTCAGCAGGAATGCCGAGACCGCAATTCATCTCGTATGCCGTC22870.2629491233112964Illumina Paired End PCR Primer 2 (96% over 30bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGCCGTC2850.038.6815945
TATGCCG2950.038.1330143
ATGCCGT3100.035.5621144
GATCGGA3850.033.8938126
ATCGGAA3750.033.59772527
AGATCGG4100.031.82711225
TCGGAAG4600.027.3894528
CCGAGAT4900.026.63084822
CGAGATC5000.026.09823223
CCTTCGT851.8822539E-723.836991
GAGATCG5650.023.49398424
CCGCAAT6800.023.49105631
CGCAATT6900.023.47667132
CTCGTAT6900.023.15060642
GTATGCC7000.022.81988345
TCTCGTA7000.022.81988341
GAGACCG7100.022.81535727
ATCTCGT7100.022.49847840
CATCTCG7100.022.49847839
AGACCGC7100.022.49847828
Produced by FastQC (version 0.11.5)