FastQCFastQC Report
Mon 6 Jun 2016
ERR1378359.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1378359.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences858532
Sequences flagged as poor quality0
Sequence length51
%GC41

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGAGCGGTTCAGCAGGAATGCCGAGATCGGAAGAGCGGTTCAGCAGGAAT44990.5240340488182154Illumina Paired End PCR Primer 2 (96% over 30bp)
CGGAAGAGCGGTTCAGCAGGAATGCCGAGACCGGTTGTTATCTCGTATGCC36540.4256102276909888Illumina Paired End PCR Primer 2 (97% over 35bp)
AAGAGCGGTTCAGCAGGAATGCCGAGACCGGTTGTTATCTCGTATGCCGTC31880.3713315287024828Illumina Paired End PCR Primer 2 (96% over 32bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATGCCGT4200.039.6413244
TATGCCG4300.038.71942543
TGCCGTC4400.037.8394445
AGATCGG5950.034.7865425
GATCGGA6000.034.12168526
ATCGGAA6100.033.56231727
TCGGAAG6450.032.0899128
GAGATCG6950.030.428724
CCGAGAT7050.029.99708622
CGAGATC7100.029.78584123
ACGCCGG450.001226996224.99757237
ATGCCGA15500.024.67502419
AATGCCG15750.024.28335818
TGCCGAG15750.024.28335820
GCCGAGA16300.023.18793321
CGGTTGT8850.022.62492232
GAATGCC17050.022.43183917
TCGTATG9000.022.24913643
TCTCGTA8900.022.2450341
CCGGTTG8900.022.2450331
Produced by FastQC (version 0.11.5)