FastQCFastQC Report
Mon 6 Jun 2016
ERR1378358.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1378358.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences545993
Sequences flagged as poor quality0
Sequence length51
%GC41

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGAGCGGTTCAGCAGGAATGCCGAGATCGGAAGAGCGGTTCAGCAGGAAT35510.6503746385026914Illumina Paired End PCR Primer 2 (96% over 30bp)
CGGAAGAGCGGTTCAGCAGGAATGCCGAGACCGTGTGTTATCTCGTATGCC31660.5798609139677615Illumina Paired End PCR Primer 2 (96% over 33bp)
AAGAGCGGTTCAGCAGGAATGCCGAGACCGTGTGTTATCTCGTATGCCGTC26260.48095854708760005Illumina Paired End PCR Primer 2 (96% over 30bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGCCGTC2650.039.0510445
ATGCCGT2750.037.63144
TATGCCG2850.036.31061643
GATCGGA4850.033.86115326
AGATCGG4900.033.51563325
ATCGGAA4700.033.50586727
TCGGAAG5100.032.20129428
CCGAGAT5300.030.9861522
CGAGATC5450.030.54610623
GAGATCG5500.030.26841424
TATCTCG6200.024.67390339
TCTCGTA6200.024.67390341
CGTATGC6300.024.63934544
AGACCGT6350.024.44533728
CTCGTAT6300.024.28225342
GACCGTG6450.024.0663429
TCGTATG6450.024.0663443
CCGTGTG6500.023.88121231
ACCGTGT6550.023.69891430
ATCTCGT6500.023.53510740
Produced by FastQC (version 0.11.5)