FastQCFastQC Report
Mon 6 Jun 2016
ERR1378357.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1378357.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1049274
Sequences flagged as poor quality0
Sequence length51
%GC41

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGAGCGGTTCAGCAGGAATGCCGAGATCGGAAGAGCGGTTCAGCAGGAAT26850.25589121621235256Illumina Paired End PCR Primer 2 (96% over 30bp)
AAGAGCGGTTCAGCAGGAATGCCGAGACCGTTGGTTATCTCGTATGCCGTC19820.18889251044055222Illumina Paired End PCR Primer 2 (96% over 32bp)
CGGAAGAGCGGTTCAGCAGGAATGCCGAGACCGTTGGTTATCTCGTATGCC19130.18231653505185488Illumina Paired End PCR Primer 2 (97% over 35bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATGCCGT2700.034.16380744
TATGCCG2950.031.26856843
TGCCGTC3000.030.74742545
GATCGGA4000.030.37245826
TCGGAAG4350.029.48029128
ATCGGAA4200.029.4618227
AGATCGG4150.029.27465825
GACCGTT5250.022.71238529
ACCGTTG5250.022.71238530
CGTTGGT5350.022.28785532
CGTATGC5450.021.87890244
TATCTCG5500.021.68000439
AATGCCG10000.021.59819418
CCGTTGG5550.021.4846931
TCGTATG5600.021.29286243
TCTCGTA5500.021.27094741
ATGCCGA10200.021.174719
CTCGTAT5550.021.07931942
AGACCGT5700.020.91930228
CCGAGAT5800.020.55862422
Produced by FastQC (version 0.11.5)