FastQCFastQC Report
Mon 6 Jun 2016
ERR1378355.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1378355.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1250855
Sequences flagged as poor quality0
Sequence length51
%GC41

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGAGCGGTTCAGCAGGAATGCCGAGATCGGAAGAGCGGTTCAGCAGGAAT30950.24743075736196443Illumina Paired End PCR Primer 2 (96% over 30bp)
CGGAAGAGCGGTTCAGCAGGAATGCCGAGACCGTACGTTATCTCGTATGCC27900.223047435554081Illumina Paired End PCR Primer 2 (96% over 33bp)
AAGAGCGGTTCAGCAGGAATGCCGAGACCGTACGTTATCTCGTATGCCGTC22720.18163576113938068Illumina Paired End PCR Primer 2 (96% over 30bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCCG2400.038.4327943
ATGCCGT2550.036.17203544
TGCCGTC2850.032.36445245
ATCGGAA4400.027.61025227
GATCGGA4500.026.99668926
AGATCGG4550.026.70002225
TCGGAAG5100.024.70285228
CGTACGT5550.023.51063232
CCGTACG5650.023.09451331
ACCGTAC5650.023.09451330
GTACGTT5650.023.09451333
CGTTATC5850.023.07409536
TACGTTA5700.022.8919334
CTCGTAT5750.022.6928742
GACCGTA5800.022.49724229
CGTATGC5950.021.93008444
TCTCGTA5950.021.93008441
TCGTATG5950.021.93008443
TATCTCG6000.021.74733439
GAGACCG6100.021.39082127
Produced by FastQC (version 0.11.5)