FastQCFastQC Report
Mon 6 Jun 2016
ERR1378354.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1378354.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences998933
Sequences flagged as poor quality0
Sequence length51
%GC41

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGAGCGGTTCAGCAGGAATGCCGAGATCGGAAGAGCGGTTCAGCAGGAAT33480.3351576131732559Illumina Paired End PCR Primer 2 (96% over 30bp)
CGGAAGAGCGGTTCAGCAGGAATGCCGAGACCGTCAGTTATCTCGTATGCC32760.32794992256737937Illumina Paired End PCR Primer 2 (96% over 33bp)
AAGAGCGGTTCAGCAGGAATGCCGAGACCGTCAGTTATCTCGTATGCCGTC25900.2592766481836119Illumina Paired End PCR Primer 2 (96% over 30bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATGCCGT2650.040.7491444
TATGCCG2800.038.5661543
ATCGGAA5000.034.6452627
GATCGGA5050.034.3022426
TGCCGTC3150.034.28102545
AGATCGG5050.033.85675425
TCGGAAG5500.032.72279428
CCGAGAT5400.032.07894522
CGAGATC5650.030.65952323
GAGATCG6000.028.8710524
AATGCCG10900.026.00561518
ATGCCGA10900.026.00561519
TGCCGAG11250.025.19655220
GCCGAGA11800.023.64083321
TCTCGTA5950.022.30787341
AGACCGT6250.021.95699528
GAATGCC13000.021.80470817
GAGACCG6300.021.78273427
CGTCAGT6200.021.77121532
CGTATGC6100.021.75931744
Produced by FastQC (version 0.11.5)