FastQCFastQC Report
Mon 6 Jun 2016
ERR1378353.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1378353.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences772058
Sequences flagged as poor quality0
Sequence length51
%GC41

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGAGCGGTTCAGCAGGAATGCCGAGATCGGAAGAGCGGTTCAGCAGGAAT58180.7535703276178733Illumina Paired End PCR Primer 2 (96% over 30bp)
AAGAGCGGTTCAGCAGGAATGCCGAGACCGGTTCTAATCTCGTATGCCGTC49120.6362216310173587Illumina Paired End PCR Primer 2 (96% over 32bp)
CGGAAGAGCGGTTCAGCAGGAATGCCGAGACCGGTTCTAATCTCGTATGCC46410.6011206411953506Illumina Paired End PCR Primer 2 (97% over 35bp)
TTCAGCAGGAATGCCGAGATCGGAAGAGCGGTTCAGCAGGAATGCCGAGAC10000.12952394768268705Illumina Paired End PCR Primer 2 (100% over 35bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGCCGTC6050.039.78983745
TATGCCG6300.038.21087643
ATGCCGT6500.037.03515644
ATCGGAA7350.035.2009427
GATCGGA7400.034.96309726
AGATCGG7550.033.97047825
TCGGAAG7900.033.8893828
CGAGATC8150.031.46958423
CCGAGAT8300.030.90085822
GAGATCG8850.028.98046324
AATGCCG18700.027.31039818
ATGCCGA18900.027.14043419
TGCCGAG18900.026.90235920
GAATGCC19900.025.55048417
GCCGAGA19550.025.43251421
GGAATGC20700.024.56302616
CTCGTAT10050.023.95308739
ACCGGTT10100.023.83450527
CGTATGC10100.023.83450541
CGGTTCT10100.023.83450529
Produced by FastQC (version 0.11.5)