FastQCFastQC Report
Mon 6 Jun 2016
ERR1378352.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1378352.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1233392
Sequences flagged as poor quality0
Sequence length51
%GC41

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGAGCGGTTCAGCAGGAATGCCGAGATCGGAAGAGCGGTTCAGCAGGAAT51740.4194935592252909Illumina Paired End PCR Primer 2 (96% over 30bp)
AAGAGCGGTTCAGCAGGAATGCCGAGACCGATTCTGATCTCGTATGCCGTC37210.3016883521216288Illumina Paired End PCR Primer 2 (96% over 32bp)
CGGAAGAGCGGTTCAGCAGGAATGCCGAGACCGATTCTGATCTCGTATGCC19230.155911502587985Illumina Paired End PCR Primer 2 (97% over 35bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCCG5450.036.3263543
ATGCCGT5500.035.99610544
ATCGGAA7150.034.6116427
TCGGAAG7550.034.2678228
TGCCGTC5850.034.2270745
AGATCGG7550.033.37387525
GATCGGA7550.033.07589326
ATGCCGA16050.028.87538119
AATGCCG16150.028.55728518
TGCCGAG16300.028.43250820
GAGATCG9150.027.78388224
CTCGTAT7150.027.68931439
CGAGATC9250.027.48351523
GACCGAT7350.027.24195326
AGACCGA7450.027.1782725
CGTATGC7350.026.93586341
TCGTATG7350.026.93586340
ACCGATT7450.026.8762927
GAATGCC17400.026.63505417
TCTCGTA7450.026.57430838
Produced by FastQC (version 0.11.5)