FastQCFastQC Report
Mon 6 Jun 2016
ERR1378350.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1378350.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences846695
Sequences flagged as poor quality0
Sequence length51
%GC41

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGAGCGGTTCAGCAGGAATGCCGAGATCGGAAGAGCGGTTCAGCAGGAAT35610.42057647677144666Illumina Paired End PCR Primer 2 (96% over 30bp)
CGGAAGAGCGGTTCAGCAGGAATGCCGAGACCGATGCTTATCTCGTATGCC34090.40262432162703216Illumina Paired End PCR Primer 2 (97% over 35bp)
AAGAGCGGTTCAGCAGGAATGCCGAGACCGATGCTTATCTCGTATGCCGTC31220.3687278181635654Illumina Paired End PCR Primer 2 (96% over 32bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATGCCGT4150.037.94911644
TGCCGTC4250.037.056245
TATGCCG4550.035.10740343
GATCGGA5350.032.80141426
AGATCGG5400.032.49769625
ATCGGAA5400.032.49769627
TCGGAAG5650.032.25435328
CGAGATC6000.028.87295523
CCGAGAT6100.028.39962822
GAGATCG6200.028.30444524
AATGCCG13450.025.09115818
TGCCGAG13550.024.90598320
ATGCCGA13750.024.54371519
GAATGCC14450.023.66614317
GCCGAGA14550.022.88497521
GGAATGC15450.022.27997416
GACCGAT7550.021.75342629
CGATGCT7850.021.4952932
GAGACCG7700.021.32965727
CCGATGC7850.021.20868731
Produced by FastQC (version 0.11.5)