FastQCFastQC Report
Mon 6 Jun 2016
ERR1378348.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1378348.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences661632
Sequences flagged as poor quality0
Sequence length51
%GC41

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGAGCGGTTCAGCAGGAATGCCGAGATCGGAAGAGCGGTTCAGCAGGAAT34460.5208333333333333Illumina Paired End PCR Primer 2 (96% over 30bp)
CGGAAGAGCGGTTCAGCAGGAATGCCGAGACCGAACCTTATCTCGTATGCC29690.4487388759914877Illumina Paired End PCR Primer 2 (97% over 36bp)
AAGAGCGGTTCAGCAGGAATGCCGAGACCGAACCTTATCTCGTATGCCGTC27430.4145809150706133Illumina Paired End PCR Primer 2 (96% over 33bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCCG2350.039.2551243
AGATCGG4600.035.70364425
ATGCCGT2650.034.81114644
TGCCGTC2650.034.81114645
ATCGGAA4850.033.3993727
GATCGGA4950.033.17914226
TCGGAAG5050.032.07662228
GAGATCG5850.028.0746624
CCGAGAT6150.027.43681322
CGAGATC6100.026.92405923
CGTATGC5800.025.6033244
CGAACCT5800.025.60138532
TCTCGTA5750.025.43465441
ACCGAAC5900.025.16746130
CCGAACC5900.025.16746131
CTCGTAT5900.024.7880142
AGACCGA6000.024.74800528
GTATGCC6050.024.54533245
TCGTATG6050.024.54533243
GACCGAA6200.023.9496829
Produced by FastQC (version 0.11.5)