FastQCFastQC Report
Mon 6 Jun 2016
ERR1378347.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1378347.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences962841
Sequences flagged as poor quality0
Sequence length51
%GC41

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGAGCGGTTCAGCAGGAATGCCGAGATCGGAAGAGCGGTTCAGCAGGAAT32300.3354655649271271Illumina Paired End PCR Primer 2 (96% over 30bp)
CGGAAGAGCGGTTCAGCAGGAATGCCGAGACCGTTACTGATCTCGTATGCC31670.3289224285214277Illumina Paired End PCR Primer 2 (97% over 35bp)
AAGAGCGGTTCAGCAGGAATGCCGAGACCGTTACTGATCTCGTATGCCGTC27210.28260117714139715Illumina Paired End PCR Primer 2 (96% over 32bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGCCGTC3200.037.26175345
ATGCCGT3300.036.1326144
TATGCCG3350.035.59331543
ATCGGAA5250.033.425127
AGATCGG5300.033.10976825
GATCGGA5300.033.10976826
TCGGAAG5900.030.88662128
CCGAGAT6450.027.55527522
CGAGATC6950.025.57288623
GAGATCG7050.025.21014624
TGCCGAG12450.024.39505620
ATGCCGA12500.024.29747819
AATGCCG12700.023.9148418
CCGTTAC6600.022.49766331
GAATGCC13600.022.3322417
CGTATGC6750.021.99771544
TCTCGTA6750.021.99771541
GTATGCC6900.021.84555845
CTCGTAT6800.021.83596842
CGTTACT6800.021.83596832
Produced by FastQC (version 0.11.5)