FastQCFastQC Report
Mon 6 Jun 2016
ERR1378346.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1378346.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences737956
Sequences flagged as poor quality0
Sequence length51
%GC41

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGAGCGGTTCAGCAGGAATGCCGAGATCGGAAGAGCGGTTCAGCAGGAAT26680.36153917035704025Illumina Paired End PCR Primer 2 (96% over 30bp)
CGGAAGAGCGGTTCAGCAGGAATGCCGAGACCGTGACTTATCTCGTATGCC23900.32386754765866804Illumina Paired End PCR Primer 2 (96% over 33bp)
AAGAGCGGTTCAGCAGGAATGCCGAGACCGTGACTTATCTCGTATGCCGTC21710.2941909815761373Illumina Paired End PCR Primer 2 (96% over 30bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATGCCGT3000.035.2469244
GATCGGA3550.034.8561126
TATGCCG3150.033.56849743
AGATCGG3700.033.44302425
ATCGGAA3800.032.56294627
TGCCGTC3250.032.5356245
TCGGAAG4050.031.663928
CGAGATC4100.030.72902323
GAGATCG4150.029.81667324
CCGAGAT4250.029.6444722
CGTGACT6150.023.4125932
CGTATGC6200.023.22377844
TATCTCG6200.023.22377839
GTATGCC6300.023.21225745
CCGTGAC6250.023.03798931
TGCCGAG10350.022.82409520
AATGCCG10450.022.82097418
TCTCGTA6350.022.67518441
ACCGTGA6350.022.67518430
AGACCGT6400.022.49803528
Produced by FastQC (version 0.11.5)