FastQCFastQC Report
Mon 6 Jun 2016
ERR1378344.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1378344.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences803536
Sequences flagged as poor quality0
Sequence length51
%GC42

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGAGCGGTTCAGCAGGAATGCCGAGATCGGAAGAGCGGTTCAGCAGGAAT47720.5938750721809601Illumina Paired End PCR Primer 2 (96% over 30bp)
CGGAAGAGCGGTTCAGCAGGAATGCCGAGACCGCGTATTATCTCGTATGCC44080.5485752971864359Illumina Paired End PCR Primer 2 (96% over 33bp)
AAGAGCGGTTCAGCAGGAATGCCGAGACCGCGTATTATCTCGTATGCCGTC41740.5194540132613847Illumina Paired End PCR Primer 2 (96% over 30bp)
TTCAGCAGGAATGCCGAGATCGGAAGAGCGGTTCAGCAGGAATGCCGAGAC8240.10254674339419764Illumina Paired End PCR Primer 2 (100% over 35bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCCG5550.038.91396743
ATGCCGT5600.038.5665244
TGCCGTC5550.038.50861445
ATCGGAA7250.032.27175527
AGATCGG7250.031.9614525
GATCGGA7500.031.49599326
TCGGAAG8000.029.80870628
GAGATCG7900.029.3317124
CGAGATC8050.028.78515823
CCGAGAT8200.028.258622
ACCGCTC507.161964E-526.99656513
AATGCCG17550.026.15051818
ATGCCGA18000.025.37176919
CTTCCGA450.001227143324.99681919
TTCCGAT450.001227143324.99681920
TGCCGAG18350.024.88784220
GCCGAGA18400.024.33114221
GGAATGC19400.023.77274716
CCGCGTA9150.023.35768128
CGCGTAT9150.023.35768129
Produced by FastQC (version 0.11.5)