FastQCFastQC Report
Mon 6 Jun 2016
ERR1378343.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1378343.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences714460
Sequences flagged as poor quality0
Sequence length51
%GC41

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGAGCGGTTCAGCAGGAATGCCGAGATCGGAAGAGCGGTTCAGCAGGAAT33840.47364443075889484Illumina Paired End PCR Primer 2 (96% over 30bp)
CGGAAGAGCGGTTCAGCAGGAATGCCGAGACCGGCTATTATCTCGTATGCC25600.35831257173249725Illumina Paired End PCR Primer 2 (96% over 33bp)
AAGAGCGGTTCAGCAGGAATGCCGAGACCGGCTATTATCTCGTATGCCGTC25300.35411359628250705Illumina Paired End PCR Primer 2 (96% over 30bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCCG2400.037.4962743
TGCCGTC2550.036.1728745
ATGCCGT2500.035.99641844
TCGGAAG5450.032.1986328
AGATCGG5250.031.7111325
ATCGGAA5300.031.4119727
GATCGGA5450.030.5474226
GGTCGGA300.00514847529.99701526
GTCGGAA300.00514847529.99701527
CCGAGAT6150.028.16792922
GAGATCG6200.027.21503324
CGAGATC6300.026.78304923
AATGCCG10550.025.58986918
ATGCCGA11050.024.43195719
TGCCGAG11200.024.10474620
CTCGTAT5000.023.84762842
CGGCTAT5050.023.61151132
CGTATGC5200.022.9304144
TCTCGTA5200.022.9304141
TATCTCG5300.022.92224739
Produced by FastQC (version 0.11.5)