FastQCFastQC Report
Mon 6 Jun 2016
ERR1378341.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1378341.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences682662
Sequences flagged as poor quality0
Sequence length51
%GC41

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGGAAGAGCGGTTCAGCAGGAATGCCGAGACCGTCGATTATCTCGTATGCC28890.42319625231813107Illumina Paired End PCR Primer 2 (96% over 33bp)
AAGAGCGGTTCAGCAGGAATGCCGAGACCGTCGATTATCTCGTATGCCGTC25890.3792506394086678Illumina Paired End PCR Primer 2 (96% over 30bp)
AAGAGCGGTTCAGCAGGAATGCCGAGATCGGAAGAGCGGTTCAGCAGGAAT25140.36826423618130205Illumina Paired End PCR Primer 2 (96% over 30bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGCCGTC3450.037.1698645
ATGCCGT3750.034.79620444
TATGCCG3750.034.1962743
ATCGGAA3950.034.1734927
GATCGGA4150.033.6107926
AGATCGG4300.032.43832425
TCGGAAG4250.031.76124428
CCGAGAT4800.028.12193522
GAGATCG4950.027.72424724
CGAGATC4900.027.54801423
GCACCCG507.159526E-526.99705512
ATGCCGA11600.022.88543519
AATGCCG11700.022.68983318
TGCCGAG11800.022.68820620
TCGATTA7200.021.87261634
CGTCGAT7250.021.7217732
ACCGTCG7150.021.71091830
GACCGTC7200.021.5601529
CTCGTAT7100.021.54694642
GTCGATT7250.021.41145933
Produced by FastQC (version 0.11.5)