FastQCFastQC Report
Mon 6 Jun 2016
ERR1378340.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1378340.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences597134
Sequences flagged as poor quality0
Sequence length51
%GC41

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGAGCGGTTCAGCAGGAATGCCGAGATCGGAAGAGCGGTTCAGCAGGAAT32400.5425917800694652Illumina Paired End PCR Primer 2 (96% over 30bp)
CGGAAGAGCGGTTCAGCAGGAATGCCGAGACCGGTCATTATCTCGTATGCC30120.5044093955460583Illumina Paired End PCR Primer 2 (97% over 36bp)
AAGAGCGGTTCAGCAGGAATGCCGAGACCGGTCATTATCTCGTATGCCGTC27240.4561790150954392Illumina Paired End PCR Primer 2 (96% over 33bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGCCGTC3400.041.6849745
TATGCCG3450.040.4287643
ATGCCGT3650.038.2134944
GATCGGA4200.035.3518826
AGATCGG4450.033.87136525
ATCGGAA4450.033.3658227
TCGGAAG4700.032.06969528
CCGAGAT4950.030.90449122
CGAGATC5000.030.59544623
TGCGGCC300.005149047429.99553326
ACGGGTT300.005149047429.99553313
GAGATCG5200.029.418724
AATGCCG11500.025.82224318
ATGCCGA11550.025.71045919
TGCCGAG11800.025.35639420
GCCGAGA12050.024.27024521
GAATGCC12800.023.1996717
GGAATGC13200.022.6670816
CGTATGC6500.021.80444741
CGGTCAT6500.021.80444729
Produced by FastQC (version 0.11.5)